pgadue

pgadue's picture

Main Profile

Full Name and Degrees: 
Paul Gadue, Ph.D.
Member Role: 
Co-PI/Co-Investigator
Institutional affiliation: 
Children's Hospital of Philadelphia
Biography: 

Associate Professor of Pathology and Laboratory Medicine

My laboratory studies cell fate decisions, focusing on endoderm and mesoderm specification using mouse and human ES cells and iPS cells. ES/iPS cells can differentiate into all cell types in the body and can be propagated in culture almost indefinitely, generating a virtually unlimited number of cells. These unique characteristics lead to the exciting prospect of using these cells to study disease processes and developmental pathways in vitro and eventually to treat a wide variety of diseases using cell replacement therapies.

The differentiation of ES cells into a given cell type closely mimics how that cell type is formed during embryogenesis. This developmental pathway starts with the formation of the primary germ layers, mesoderm, endoderm, and ectoderm. Progressively more differentiated cell types are formed until the functional mature cell is generated. My research program focuses on understanding the molecular mechanisms that regulate endoderm and mesoderm development utilizing the in vitro differentiation of ES cells and iPS cells.

One area of interest in the lab is in investigating hematopoiesis with a focus on megakaryocyte development. We are studying the molecular pathways, which regulate megakaryopoeisis with the goal of optimizing the generation of platelets in vitro from ES/iPS cells. In addition, we are developing in vitro models of platelet disorders using iPS cells derived from patients with genetic diseases affecting platelet development and function as well as using CRISPR/Cas9 technology to both correct mutant iPS cell lines and generate patient specific mutations in wild-type lines.

The second area of interest in the lab is endoderm formation. We are studying a unique endodermal stem cell population that we have generated from human ES and iPS cells. Endoderm stem cells have the ability to be expanded in culture like ES cells and have the capability to generate many endoderm derived tissues such as liver, pancreas and intestine. We are studying the signaling and transcriptional pathways, which regulate endoderm stem cell generation and maintenance. We are also utilizing the human stem cell model to study pancreatic beta cell specification with the goal of generating functional beta cells from human ES and iPS cells. Lastly, we are using the stem cell system to model genetic forms of diabetes, using both patient specific iPS cells and genome editing to generate genetic models of these diseases.

Recent publications: 
Tiyaboonchai Amita, Cardenas-Diaz Fabian L, Ying Lei, Maguire Jean Ann, Sim Xiuli, Jobaliya Chintan, Gagne Alyssa L, Kishore Siddharth, Stanescu Diana E, Hughes Nkecha, De Leon Diva D, French Deborah L, Gadue Paul: GATA6 Plays an Important Role in the Induction of Human Definitive Endoderm, Development of the Pancreas, and Functionality of Pancreatic β Cells. Stem cell reports Feb 2017.   Maguire Jean Ann, Gagne Alyssa L, Jobaliya Chintan D, Gandre-Babbe Shilpa, Gadue Paul, French Deborah L: Generation of human control iPS cell line CHOPWT10 from healthy adult peripheral blood mononuclear cells. Stem cell research 16(2): 338-41, Mar 2016.   Maguire Jean Ann, Lu Lin, Mills Jason A, Sullivan Lisa M, Gagne Alyssa, Gadue Paul, French Deborah L: Generation of Hermansky-Pudlak Syndrome Type 1 (HPS1) induced pluripotent stem cells (iPSCs). Stem cell research 16(2): 233-5, Mar 2016. Maguire Jean Ann, Lu Lin, Mills Jason A, Sullivan Lisa M, Gadue Paul, French Deborah L: Generation of Hermansky Pudlak syndrome type 2 (HPS2) induced pluripotent stem cells (iPSCs). Stem cell research 16(2): 287-9, Mar 2016.   Maguire Jean Ann, Gagne Alyssa, Mills Jason A, Gadue Paul, French Deborah L: Generation of human control iPS cell line CHOPWT9 from healthy adult peripheral blood mononuclear cells. Stem cell research 16(1): 14-6, Jan 2016.
Address: 
Children's Hospital of Philadelphia 3615 Civic Center Blvd. CTRB, Rm. 5012 Philadelphia, PA 19104
Phone: 
267-426-9607

Copyright ©2016 NHLBI Progenitor Cell Translational Consortium.

University of Maryland School of Medicine logo

National Heart Blood and Lung Institute logo